Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone.Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways.
Department of Pathology, The First Affiliated Hospital, School of Medicine, Zhejiang University. 310003 How to cite this article: Li J, Xin J, Zhang L, Wu J, Jiang L, Zhou Q, Li J, Guo J, Cao H, Li L. Available from To clarify the precise characteristics of human hepatic progenitor cells (HPCs) for future cytotherapy in liver diseases.
Results: Cultured HPCs contained polygonal, high nucleus/cytoplasm ratio and exhibited a global gene expression profile similar (67.8%) to that of primary hepatocytes.
Among the genes with more than 20-fold higher expression in HPCs were a progenitor marker (CD90), a pentraxin-related gene (PTX3), collagen proteins (COL5A2, COL1A1 and COL4A2), cytokines (EGF and PDGFD), metabolic enzymes (CYBRD1, BCAT1, TIMP2 and PAM), a secreted protein (SPARC) and an endothelial protein C receptor (PROCR).
Moreover, eight markers (ALB, AFP, CK8, CK18, CK19, CD90, CD117 and Oval-6) previously described as HPC markers were validated by q RT-PCR and/or immunocyto/histochemistry.
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slang), plonker (slang), dong (slang), winkle (Brit.
taboo slang), John Thomas (taboo slang), weenie (U.
It is found naturally in citrus fruits and many vegetables.
Interestingly, human HPCs were also positive for the hematopoietic cell markers CD45 and CD109.
Finally, we characterized the localization of HPCs in the canals of Hering and periportal areas with six previously described markers (Oval-6, CK8, CK18, CK19, CD90 and CD117) and two potential markers (CD45 and CD109).
Conclusion: The human HPCs are highly similar to primary hepatocytes in their transcriptional profiles.